WebProduct description. Hieff NGS™ mRNA Isolation Master Kit is a magnetic bead kit specially for mRNA purification. The mRNA Capture beads are micrometer-sized paramagnetic microspheres coupled with Oligo dT (poly A) tails, which can be used to isolate mRNA from 10 ng to 4 μg of complete RNA by binding to mRNA with poly A tails. WebRNA Isolation & Enrichment. Studies that require the isolation of eukaryotic mRNA often take advantage of the polyadenine tail typically present at the 3’ end. One approach involves the use affinity matrices; poly-T oligonucleotides that hybridize to the complementary poly-A tails (1). Enrichment of miRNA and siRNAs can also be achieved using ...
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